Application

Immunohistochemistry Analysis: 0.5 µg/mL of this antibody detected PEG (methoxy)-BSA in injected mouse liver and kidney
ELISA Analysis: Multiple concentrations of this antibody was used to detect three different BSA-PEG conjugates (5K, 40K and 40K branched)

Research CategorySignaling

Research Sub CategoryEpitope Tags

Anti-PEG (methoxy group), clone RM105 is an antibody against PEG (methoxy group) for use in WB, IH, ELISA.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Polyethylene glycol (PEG) is a polyether compound with many applications from industrial manufacturing to medicine. PEG is also known as polyethylene oxide (PEO) or polyoxyethylene (POE), depending on its molecular weight. PEG is a family of long chain polymers attached to a glycerine backbone. Some of the properties of PEG are nonionic, nontoxic, biocompatible, strongly hydrophilic polymer, which has a large exclusion volume in aqueous solution. PEGylation is the act of covalently coupling a PEG structure to another larger molecule, for example, a therapeutic protein, which is then referred to as a PEGylated protein. The covalent attachment of PEG (PEGylation) is commonly used to modify a variety of proteins and drugs. Some of the therapeutical benefits of PEGylated molecules include increased bioavailability, increased blood circulation of the drug, optimized pharmacokinetics, decreased immunogenicity, and decreased frequency of administration. Additional benefits of PEGylated molecules include decelerated renal excretion, improved stability towards proteolysis, increased solubility of the biopharmaceutical in aqueous solutions, increased hydrodynamic radius, reduced immunogenicity and proteolytic cleavage. This antibody reacts to the terminal methoxy group of Polyethylene glycol.

Immunogen

recombinant protein corresponding to All PEG (methoxy group).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein A purified

Purified rabbit monoclonal IgG in PBS buffer containing 1% BSA and 0.09% sodium azide

Quality

Evaluated by Western Blotting on recombinant methoxyPEG conjugated BSA

Western Blotting Analysis: A 1:1;000 dilution of this antibody detected PEG (methoxy group) on conjugated recombinant BSA

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

BSA is 66 kD, methoxyPEG is 5 kD. Additional bands above 66 kD is due to different levels of conjugation and dimerization.